6x DNA Loading Dye Buffer (Ficoll based)
Description and features:
6x DNA Loading Dye Buffer can be used for loading DNA samples (PCR products, restriction fragments) on agarose gel.
6x DNA Loading Dye Buffer is premixed ready-to-use marker dye containing Orange G, Tris-HCl, EDTA, Ficoll-400 for easy visual monitoring during the electroforesis.
Orange G dye is visible on agarose gels and it runs faster than bromphenol blue and the smallest detectable DNA fragment (approx. 51 bp).
Orange G dye is a better indicator to prevent the risk of your DNA running off the gel and does not influence band intensity on pictures taken under the UV.
No interaction with the borate on TBE gels, altering local pH due Ficoll.
Mix 1 volume of Dye with 5 volumes of your DNA sample and load into the gel (final Ficoll is 4.2%). Also, DNA Loading Dye works like 10x - mix 1 volume Dye with 9 volume of your sample (final Ficoll is 2.5%).
Storage: Store at RT, +4C, and for longer period at -20C recommended
Some applications of this product may be protected and require a license which is not provided by the purchase of this product. US DNA recommends all users to obtain licenses as necessary. |
